OBJECTIVES: To give insights into modern research trends in biophotonics. To provide in particular an overview of the various optical techniques available for biomedical imaging and detection, giving their characteristics and highlighting their advantages and drawbacks.

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INTRODUCTION TO OPTICAL IMAGING OF BIOLOGICAL MEDIA
A. Dubois, Lab. Charles Fabry, Institut d’Optique Graduate School
1,5h

INTRODUCTION TO CELL BIOLOGY
Cellular components, DNA, RNA, proteins
C. Bouzigues, Lab. d'Optique et Biosciences, Ecole Polytechnique
1,5h

OPTICAL MICROSCOPY
Fluorescence microscopy, confocal microscopy. Full-field imaging techniques. Organic/inorganic fluorophores
C. Bouzigues – 3h

FLUORESCENCE TECHNIQUES
Single-molecule tracking, Fluorescence Recovery After Photobleaching (FRAP), Fluorescence Correlation and Cross-Correlation (FCS, FCCS), Fluorescence lifetime imaging (FLIM), Fluorescence Resonant Energy Transfer (FRET).
C. Bouzigues – 3h

SUPER-RESOLUTION IMAGING
Total Internal Reflection Fluorescence microscopy (TIRF), 4π microscopy, Stimulated Emission Depletion microscopy (STED), Stochastic Optical Reconstruction Microscopy (STORM), PhotoActivated Localization Microscopy (PALM).
C. Bouzigues - 1h30

OPTICAL TWEEZERS
Single molecule manipulation
N. Westbrook - Lab. Charles Fabry, Institut d’Optique Graduate School
1h30

DNA and PROTEIN MICRO-ARRAYS
Readout techniques: fluorescence and Surface Plasmon Resonance, Biochip specifications and realizations, Data processing and interpretation
H. Benisty, Lab. Charles Fabry, Institut d’Optique Graduate School
3h

NON-LINEAR MICROSCOPY
Two-photon excitation fluorescence microscopy
Harmonic generation microscopy
Coherent Anti-Stokes Raman Scattering (CARS) microscopy
E. Beaurepaire Lab. d'Optique et Biosciences, Ecole Polytechnique
3h

OPTICAL COHERENCE TOMOGRAPHY
Time-domain / frequency-domain OCT
Full-field OCT
Applications
A. Dubois, Lab. Charles Fabry, Institut d’Optique Graduate School
3h

Modalités d'évaluation : Examen écrit

Dernière mise à jour : Tuesday 23 January 2018